Journal: American Journal of Physiology - Renal Physiology

Article Title: The mechanosensitive BKα/β1 channel localizes to cilia of principal cells in rabbit cortical collecting duct (CCD)

doi: 10.1152/ajprenal.00256.2016

Figure Lengend Snippet: Antibodies (Abs) used for immunoperfusion

Article Snippet: 3D reconstructions were generated using Leica Application Suite (LAS AF) software. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Primary Abs Dilution Provider Secondary Abs Dilution Provider Goat IgG anti-maxi-Kβ1 (N-15) 1:100 Santa Cruz Biotechnology (sc-14749) A488-rabbit IgG anti-goat IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11078","term_id":"490929","term_text":"A11078"}} A11078 ) Mouse IgG2a anti-maxi-Kβ4 1:100 Stress Marq Biosciences (SMC-38D) A488-goat IgG anti-mouse IgG2a 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A21131","term_id":"514092","term_text":"A21131"}} A21131 ) Mouse IgG2b anti-acetylated α-tubulin (6-11B-1) 1:1,000 Abcam (ab24610) A488-goat IgG anti-mouse IgG 1:1,000 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11029","term_id":"492395","term_text":"A11029"}} A11029 ) Goat IgG anti-aquaporin-2 (C-17) 1:100 Santa Cruz Biotechnology (sc-9882) A488-rabbit IgG anti-goat IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11078","term_id":"490929","term_text":"A11078"}} A11078 ) Mouse IgG2a anti-polycystin-2 (YCE2) 1:100 Santa Cruz Biotechnology (sc-47734) A647-goat IgG anti-mouse IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A21236","term_id":"583506","term_text":"A21236"}} A21236 ) Rabbit anti-pendrin 1:2,000 Gift from Dr. S. Wall, Emory University School of Medicine A488-goat IgG anti-rabbit IgG 1:500 Molecular Probes ( {"type":"entrez-nucleotide","attrs":{"text":"A11008","term_id":"492390","term_text":"A11008"}} A11008 ) Open in a separate window Antibodies (Abs) used for immunoperfusion The effect of K + intake on relative BKα expression in the cilia was determined by analysis of CCDs isolated from HK ( n = 4)- or LK ( n = 4)-fed rabbits and immunoperfused simultaneously with Abs directed against BKα and acetylated α-tubulin (Ac α-tubulin), followed by appropriate secondary Abs ( ).

Techniques:

Journal: Molecular Pain

Article Title: Sigma-1 receptors and progesterone metabolizing enzymes in nociceptive sensory neurons of the female rat trigeminal ganglia: A neural substrate for the antinociceptive actions of progesterone

doi: 10.1177/17448069211069255

Figure Lengend Snippet: Antibodies used for western blotting and fluorescent immunohistochemistry.

Article Snippet: The tissue sections were then washed with 0.1 m PBS three times and then incubated with the corresponding fluorescent-conjugated secondary antibody goat anti-rabbit Alexa-488 or 568 (1:300; Molecular Probes, Eugen, OR, USA), goat anti-mouse Alexa-488 or 568 (1:300; Molecular Probes), or donkey anti-goat Alexa-647 (1:300; Molecular Probes) for 90 min, rinsed, then subjected to a second round of primary immune-labeling overnight at room temperature with primary antibody for Isolectin IB4 (1:500; Fischer Scientific, I21414) in 0.1 m m CaCl 2 , rabbit anti-TRPV1 (1:1000; Alomone, ACC-030), goat anti-Nav1.8 (1:1000; Alomone, SCN10 A), goat anti-CGRP (1:1000; Abcam, AB36001) or rabbit anti-CGRP (1:1000; Immunostar 24,112), guinea pig anti-PGP9.5 (1:1000; Millipore, AB5898), or chicken anti-NF200 (1:1000; Abcam, AB4680).

Techniques: Western Blot, Immunohistochemistry